Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Polarization Filter, 700 nm BW 60 nm, Vertical
This fluorescence polarization filter in a holder enables near-infrared fluorescence polarization measurements centered at 700 nm and installs into the filter slot of compatible multi-mode microplate readers to support FP assays.
- 700 nm center wavelength with 60 nm bandwidth.
- Fluorescence polarization optical design for polarization-based measurements.
- Vertical polarization orientation for polarization detection.
- Mounted in a holder for insertion into reader filter slots.
- Intended for fluorescence polarization assays and near-IR detection workflows.
Revvity Health Sciences Inc HTRF Human Total MET Detection Kit, Control Lysate
HTRF Human Total MET Detection Kit, Control Lysate
Molecular Devices Filter Lens, 450 nm, For Microplate Readers
The Molecular Devices 450 nm filter lens (MPN 6500-0007) is an optical replacement filter designed for compatible microplate readers. It provides a center wavelength of 450 nm for use in fluorescence and absorbance detection applications and serves as an interchangeable detection filter for select filter-based plate readers.
- Provides center wavelength of 450 nm.
- Intended for fluorescence and absorbance detection.
- Replacement optical filter lens for compatible microplate readers.
- Compatible with select filter-based plate reader models.
Revvity Health Sciences Inc HTRF Human Amyloid-&beta1; 42 Detection Kit Standard
HTRF Human Amyloid-&beta1; 42 Detection Kit Standard
Molecular Devices Fluorescence Polarization Filter, 535 nm, 25 nm, Vertical
Fluorescence polarization filter optimized for fluorescence polarization assays. The filter has a 535 nm center wavelength with a 25 nm bandwidth and a vertical polarization orientation. It includes a holder for mounting in compatible microplate readers and is intended for use with FP measurement configurations.
- Optimized for fluorescence polarization measurements.
- Center wavelength 535 nm, bandwidth 25 nm.
- Vertical polarization orientation for polarization-based assays.
- Supplied with a holder for secure mounting in reader filter positions.
- Compatible with SpectraMax and similar microplate readers that accept filter holders.
Revvity Health Sciences Inc HTRF SARS-CoV2 nucleocapsid Detection Kit, 10,000 Assay Points
HTRF SARS-CoV2 nucleocapsid Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD7 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total BRD7 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc AlphaScreen mAb Anti-DIG Donor Beads, 5 mg
AlphaScreen mAb Anti-DIG Donor Beads, 5 mg
Molecular Devices Filter Lens, 560 nm, EMax and VMax Compatible
The 560 nm filter lens is an optical replacement filter designed for use with EMax and VMax microplate readers. It provides centered transmission at 560 nanometers for measurements that require that wavelength and is supplied as an individual replacement lens.
- Optical center wavelength 560 nm.
- Compatible with EMax and VMax microplate readers.
- Sold as a single replacement filter lens.
- Intended for fluorescence and absorbance applications requiring a 560 nm bandpass.
Revvity Health Sciences Inc HTRF Human XIAP / BIR2 Binding Kit Standard
HTRF Human XIAP / BIR2 Binding Kit Standard
Revvity Health Sciences Inc HTRF CD16a (FcgRIIIa, V158) Cellular Binding Kit, 1,000 Assay Points
HTRF CD16a (FcgRIIIa, V158) Cellular Binding Kit, 1,000 Assay Points
Revvity Health Sciences Inc HTRF Estradiol Detection Kit Standard
HTRF Estradiol Detection Kit Standard
Revvity Health Sciences Inc HTRF AAV9 Detection Kit, 10,000 Assay Points
HTRF AAV9 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATG14 Detection Kit, 500 Assay Points
HTRF Human Total ATG14 Detection Kit, 500 Assay Points