Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Filter Lens, 450 nm, For Microplate Readers
The Molecular Devices 450 nm filter lens (MPN 6500-0007) is an optical replacement filter designed for compatible microplate readers. It provides a center wavelength of 450 nm for use in fluorescence and absorbance detection applications and serves as an interchangeable detection filter for select filter-based plate readers.
- Provides center wavelength of 450 nm.
- Intended for fluorescence and absorbance detection.
- Replacement optical filter lens for compatible microplate readers.
- Compatible with select filter-based plate reader models.
Molecular Devices Photometric Filter, 560 nm, Filter Slide Compatible
Replacement photometric filter for FilterMax F3 and F5 multi-mode microplate readers. Provides a fixed-wavelength photometric option centered at 560 nm for absorbance and ELISA assays. Sold as a single filter unit for installation in multi-position filter slides.
- Fixed center wavelength of 560 nm.
- Intended for photometric (absorbance) assays and ELISA.
- Sold individually as a single filter unit.
- Designed for installation in multi-position filter slides of microplate readers.
- Used to restore or expand fixed-wavelength reading options.
Molecular Devices Fluorescence Excitation Filter, 535 nm, 25 nm bandwidth
Molecular Devices 535 nm fluorescence excitation filter (MPN 6590-0057) is a bandpass excitation filter centered at 535 nm with a 25 nm bandwidth, intended for installation in compatible microplate reader filter slides to provide selective excitation for fluorescence assays.
- Excitation bandpass centered at 535 nm with a 25 nm bandwidth.
- Optimized for fluorescence assay excitation to improve signal specificity.
- Form factor designed for installation in compatible microplate reader filter slides.
- Intended for use with multi-mode microplate readers that accept filter slides.
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm BW
Molecular Devices fluorescence polarization filter specified for a 635 nm center wavelength with a 35 nm bandwidth. It is designed for fluorescence polarization assays in microplate and ELISA readers and is supplied with a holder for insertion into compatible filter modules.
- Suitable for fluorescence polarization measurements.
- 635 nm center wavelength, 35 nm bandwidth.
- Polarized horizontal orientation.
- Includes holder for filter module insertion.
- Intended for microplate and ELISA reader applications.
Molecular Devices Fluorescence Polarization Filter, 535 nm, 25 nm, Vertical
Fluorescence polarization filter optimized for fluorescence polarization assays. The filter has a 535 nm center wavelength with a 25 nm bandwidth and a vertical polarization orientation. It includes a holder for mounting in compatible microplate readers and is intended for use with FP measurement configurations.
- Optimized for fluorescence polarization measurements.
- Center wavelength 535 nm, bandwidth 25 nm.
- Vertical polarization orientation for polarization-based assays.
- Supplied with a holder for secure mounting in reader filter positions.
- Compatible with SpectraMax and similar microplate readers that accept filter holders.
Molecular Devices Fluorescence Polarization Filter, Polarized Horizontal, 300-750 nm
Molecular Devices polarized horizontal fluorescence polarization filter for use in fluorescence polarization assays on compatible multi-mode microplate readers. The filter transmits across a broad UV-visible range and installs in a standard filter slide to enable polarized detection.
- Polarized horizontal orientation.
- Wavelength range 300-750 nm.
- Enables fluorescence polarization measurements.
- Installs in a standard filter slide for compatible multi-mode readers.
- Broad UV-visible transmission for diverse fluorophores.
Molecular Devices Polarization Emission Filter, Visible, Pair, For FilterMax Readers
Replacement polarization emission filter set for fluorescence polarization measurements in multi-mode microplate readers. Supplied as a pair (quantity 2), these emission filters are intended to enable polarization-based fluorescence detection and improve signal discrimination in applicable assays.
- Replacement emission filters for fluorescence polarization assays.
- Visible emission band, vendor designation "Visible".
- Sold as a pair (quantity 2) for filter-based reader configurations.
- Intended for compatible multi-mode microplate readers.
- Helps improve polarization signal discrimination and assay reproducibility.
Molecular Devices Microplate Reader, 6-1536-Well, Dual PMT, User-Upgradeable
The SpectraMax Paradigm multi-mode microplate reader from Molecular Devices is a user-upgradeable detection platform for absorbance, fluorescence, time-resolved fluorescence, fluorescence polarization, and luminescence assays. It supports 6- to 1536-well plates and modular detection cartridges for configurable optics.
- User-upgradeable detection cartridges for configurable optics and modes.
- Supports 6- to 1536-well plate formats.
- Dual photomultiplier tubes for enhanced sensitivity and throughput.
- Wide absorbance range 230-1000 nm and fluorescence excitation/emission coverage.
- Integrated temperature control, plate shaking, and robotic compatibility.
- High dynamic range greater than six decades for quantitative assays.
- Compact footprint: 39 cm W x 45 cm H x 63 cm D.
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays
Molecular Devices Photometric Filter, Absorbance 590 nm, FilterMax Compatible
The AD 340 photometric filter provides a 590 nm center wavelength bandpass for absorbance (photometric) measurements in multimode microplate readers. Supplied as an accessory by Molecular Devices (MPN 6590-0023), the filter installs in a reader's filter holder to enable targeted optical selection for absorbance assays and routine photometric measurements.
- Central wavelength 590 nm for absorbance measurements.
- Designed for photometric microplate assays.
- Installs in a standard filter holder for quick replacement.
- Provides consistent optical selection for repeatable readings.
Molecular Devices Microplate Reader, Multi-Mode, Monochromator, Temperature Control
The SpectraMax M5e multi-mode microplate reader provides flexible, high-performance detection for absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF assays across 6-384 well plates and cuvettes. It features a tunable monochromator with 1 nm spectral resolution, temperature control, and adaptive detector optimization for broad dynamic range.
- Supports absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF assays.
- Monochromator with 1 nm wavelength selection for spectral scanning and up to six wavelengths per read.
- Temperature control from ambient +2°C to 60°C for incubated assays.
- Automated detector optimization and absorbance normalization for extended dynamic range and improved accuracy.
- Compatible with GxP-capable data acquisition and validation workflows for regulated environments.
Molecular Devices Absorbance Filter, 515 nm, 8 nm
Absorbance bandpass filter suitable for microplate absorbance assays. Centered at 515 nm with an 8 nm bandwidth, this optical filter installs in filter slide slots and provides narrowband absorbance detection for plate-based assays. Compatible with FilterMax F3 and F5 multimode microplate readers.
- Center wavelength 515 nm.
- Bandwidth 8 nm.
- Filter type Absorbance bandpass.
- Application Microplate absorbance measurements.
- Form factor Slide-mounted filter for filter-based readers.
Revvity Health Sciences Inc HTRF Mouse Total STING Detection Kit, 500 Assay Points
HTRF Mouse Total STING Detection Kit, 500 Assay Points
Molecular Devices Fluorescence Polarization Filters, 590 nm, 20 nm
Fluorescence polarization emission filter set for fluorescence polarization and related fluorescence detection assays. The set includes two polarized emission filters and a holder for mounting in compatible filter-based fluorescence instruments.
- Set of two polarized emission filters for fluorescence polarization measurements.
- Center wavelength 590 nm, bandwidth 20 nm.
- One vertical and one horizontal polarized filter for polarization assays.
- Includes holder for secure filter mounting.
- Designed for use in fluorescence polarization and related fluorescence detection workflows.